Villa Agnolaccio is barely 65km from Galileo Galilei International Airport of Pisa and 30km from Amerigo Vespucci Airport of Florence. For both the origins is available the highway to the exit of Pistoia on the A11 or the railway both from Pisa and Florence to Pistoia Railway Station.

Villa Agnolaccio can be reached in about 5 minutes by car following Via Provinciale Lucchese in direction of Montecatini Terme then, once arrived in Spazzavento, taking Via del Poggio and, after the ancient Convento di Giaccherino, continuing on Via Acciaio e Agnolaccio.